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Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 4 (27/11/2008)

By Zehr, J. P.

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Book Id: WPLBN0004021033
Format Type: PDF Article :
File Size: Pages 33
Reproduction Date: 2015

Title: Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 4 (27/11/2008)  
Author: Zehr, J. P.
Volume: Vol. 5, Issue 4
Language: English
Subject: Science, Ocean, Science
Collections: Periodicals: Journal and Magazine Collection, Copernicus GmbH
Publication Date:
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications


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Hewson, I., Moisander, P. H., & Zehr, J. P. (2008). Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 4 (27/11/2008). Retrieved from

Description: Department of Ocean Sciences, University of California Santa Cruz, 1156 High Street E&MS D446, Santa Cruz, CA 95064, USA. The study of marine microorganisms using molecular biological techniques is now widespread in the ocean sciences. These techniques target nucleic acids which record the evolutionary history of microbes, and encode for processes which are active in the ocean today. Here we review some of the most commonly used molecular biological techniques. Molecular biological techniques permit study of the abundance, distribution, diversity, and physiology of microorganisms in situ. These techniques include the polymerase chain reaction (PCR) and reverse-transcriptase PCR, quantitative PCR, whole assemblage fingerprinting approaches (based on nucleic acid sequence or length heterogeneity), oligonucleotide microarrays, and high-throughput shotgun sequencing of whole genomes and gene transcripts, which can be used to answer biological, ecological, evolutionary and biogeochemical questions in the ocean sciences. Moreover, molecular biological approaches may be deployed on ocean sensor platforms and hold promise for tracking of organisms or processes of interest in near-real time.

Molecular biology techniques and applications for ocean sensing

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