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Technical Note: How Long Can Seawater Oxygen Samples Be Stored Before Titration? : Volume 11, Issue 5 (29/10/2014)

By Lankhorst, M.

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Book Id: WPLBN0004020811
Format Type: PDF Article :
File Size: Pages 13
Reproduction Date: 2015

Title: Technical Note: How Long Can Seawater Oxygen Samples Be Stored Before Titration? : Volume 11, Issue 5 (29/10/2014)  
Author: Lankhorst, M.
Volume: Vol. 11, Issue 5
Language: English
Subject: Science, Ocean, Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Copernicus GmbH
Publication Date:
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications


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Send, U., Nam, S. H., Chavez, G., & Lankhorst, M. (2014). Technical Note: How Long Can Seawater Oxygen Samples Be Stored Before Titration? : Volume 11, Issue 5 (29/10/2014). Retrieved from

Description: Scripps Institution of Oceanography, 9500 Gilman Drive, La Jolla, CA 92093, USA. The concentration of dissolved oxygen in seawater is routinely measured using a standardized titration method that involves analysis shortly after the water sample has been collected. However, none of the existing procedural documents are specific about how soon after collection the titration has to be done. Here, we report on a small number of samples where duplicates were collected and one batch was titrated within days after collection, while the other batch was stored for several weeks before titration. In addition, for a subset of the samples a third batch was taken that was stored like the others but with a particular chemical already added before storage. Comparison between the batches confirms that there is no significant difference between the ones that were stored and the ones that were analyzed sooner, indicating that a month-long storage period is acceptable. The implication of this is that such oxygen samples do not necessarily have to be analyzed while still on the ship; instead, it is possible to transport them ashore for analysis there.

Technical Note: How long can seawater oxygen samples be stored before titration?

AADI-4831 reference: Oxygen Optode 4831/4831F, Aanderaa Data Instruments AS, Bergen, Norway, specification sheet, document number D403, 2012.; Carpenter, J. H.: The accuracy of the Winkler method for dissolved oxygen analysis, Limnol. Oceanogr., 10, 135–140, doi:10.4319/lo.1965.10.1.0135, 1965a.; Carpenter, J. H.: The Chesapeake Bay Institute technique for the Winkler dissolved oxygen method, Limnol. Oceanogr., 10, 141–143, doi:10.4319/lo.1965.10.1.0141, 1965b.; Champenois, W. and Borges, A. V.: Seasonal and interannual variations of community metabolism rates of a Posidonia oceanica seagrass, Limnol. Oceanogr., 57, 347–361, 2012.; Clark Jr., L. C., Wolf, R., Granger, D., and Taylor, Z.: Continuous recording of blood oxygen tensions by polarography, J. Appl. Physiol., 6, 189–193, 1953.; Cronin, M. F., Weller, R. A., Lampitt, R. S., and Send, U.: Ocean reference stations, in: Earth Observation, InTech, ISBN: 978-953-307-973-8, available at: (last access: 23 October 2014), 2012.; Dickson, A.: Determination of dissolved oxygen in sea water by Winkler titration, available at: (last access: 23 October 2014), 1996.; Klimant, I., Meyer, V., and Kühl, M.: Fiber-optic oxygen microsensors, a new tool in aquatic biology, Limnol. Oceanogr., 40, 1159–1165, 1995.; Langdon, C.: Determination of dissolved oxygen in seawater by Winkler titration using the amperometric technique, in: The GO-SHIP Repeat Hydrography Manual: A Collection of Expert Reports and Guidelines, edited by: Hood, E. M., Sabine, C. L., and Sloyan, B. M., IOCCP Report Number 14, ICPO Publication Series Number 134, available at: (last access: 23 October 2014), 2010.; SBE-63 reference: SBE 63 User's Manual, Sea-Bird Electronics, Inc, Bellevue, WA, USA, version #008, 2014.; Winkler, L. W.: Die Bestimmung des im Wasser gelösten Sauerstoffes, Ber. Dtsch. Chem. Ges., 21, 2843–2854, 1888.


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