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An Optimised Method for Correcting Quenched Fluorescence Yield : Volume 11, Issue 3 (09/05/2014)

By Biermann, L.

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Book Id: WPLBN0004020777
Format Type: PDF Article :
File Size: Pages 22
Reproduction Date: 2015

Title: An Optimised Method for Correcting Quenched Fluorescence Yield : Volume 11, Issue 3 (09/05/2014)  
Author: Biermann, L.
Volume: Vol. 11, Issue 3
Language: English
Subject: Science, Ocean, Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Copernicus GmbH
Historic
Publication Date:
2014
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications

Citation

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Guinet, C., Boehme, L., Brierley, A., Bester, M., & Biermann, L. (2014). An Optimised Method for Correcting Quenched Fluorescence Yield : Volume 11, Issue 3 (09/05/2014). Retrieved from http://worldlibrary.net/


Description
Description: Sea Mammal Research Unit, Scottish Oceans Institute, St. Andrews, UK. Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. Previously, this has been done using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, the assumption of homogeneity is not robust in oceanic regimes that support deep chlorophyll maxima. To account for these features, we correct from the limit of the euphotic zone, defined as the depth at which light is at ~1% of the surface value. This method was applied to fluorescence data collected by eleven animal-borne fluorometers deployed in the Southern Ocean over four austral summers. Six tags returned data showing evidence of deep chlorophyll features. Using the depth of the euphotic layer, quenching was corrected without masking subsurface fluorescence signals.

Summary
An optimised method for correcting quenched fluorescence yield

Excerpt
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