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On Dithiothreitol (Dtt) as a Measure of Oxidative Potential for Ambient Particles: Evidence for the Importance of Soluble Transition Metals : Volume 12, Issue 5 (03/05/2012)

By Charrier, J. G.

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Book Id: WPLBN0003996062
Format Type: PDF Article :
File Size: Pages 34
Reproduction Date: 2015

Title: On Dithiothreitol (Dtt) as a Measure of Oxidative Potential for Ambient Particles: Evidence for the Importance of Soluble Transition Metals : Volume 12, Issue 5 (03/05/2012)  
Author: Charrier, J. G.
Volume: Vol. 12, Issue 5
Language: English
Subject: Science, Atmospheric, Chemistry
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Copernicus GmbH
Historic
Publication Date:
2012
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications

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Anastasio, C., & Charrier, J. G. (2012). On Dithiothreitol (Dtt) as a Measure of Oxidative Potential for Ambient Particles: Evidence for the Importance of Soluble Transition Metals : Volume 12, Issue 5 (03/05/2012). Retrieved from http://worldlibrary.net/


Description
Description: Department of Land, Air and Water Resources, University of California, Davis, 1 Shields Ave., Davis, CA, 95616, USA. The rate of consumption of dithiothreitol (DTT) is increasingly used to measure the oxidative potential of particulate matter (PM), which has been linked to the adverse health effects of PM. While several quinones are known to be very reactive in the DTT assay, it is unclear what other chemical species might contribute to the loss of DTT in PM extracts. To address this question, we quantify the rate of DTT loss from individual redox-active species that are common in ambient particulate matter. While most past research has indicated that the DTT assay is not sensitive to metals, our results show that seven out of the ten transition metals tested do oxidize DTT, as do three out of the five quinones tested. While metals are less efficient at oxidizing DTT compared to the most reactive quinones, concentrations of soluble transition metals in fine particulate matter are generally much higher than those of quinones. The net result is that metals appear to dominate the DTT response for typical ambient PM2.5 samples. Based on particulate concentrations of quinones and soluble metals from the literature, and our measured DTT responses for these species, we estimate that for typical fine particle samples approximately 80% of DTT loss is from transition metals (especially copper and manganese), while quinones account for approximately 20%. We find a similar result for DTT loss measured in a small set of PM2.5 samples from the San Joaquin Valley of California. Because of the important contribution from metals, we also tested how the DTT assay is affected by EDTA, a chelator that is sometimes used in the assay. EDTA significantly suppresses the response from both metals and quinones; we therefore recommend that EDTA should not be included in the DTT assay.

Summary
On dithiothreitol (DTT) as a measure of oxidative potential for ambient particles: evidence for the importance of soluble transition metals

Excerpt
Ayres, J. G., Borm, P., Cassee, F. R., Castranova, V., Donaldson, K., Ghio, A., Harrison, R. M., Hider, R., Kelly, F., Kooter, I. M., Marano, F., Maynard, R. L., Mudway, I., Nel, A., Sioutas, C., Smith, S., Baeza-Squiban, A., Cho, A., Duggan, S., and Froines, J.: Evaluating the toxicity of airborne particulate matter and nanoparticles by measuring oxidative stress potential – a workshop report and consensus statement, Inhal. Toxicol., 20, 75–99, 2008.; Biorad: Chelex 100 and Chelex 20 chelating ion exchange resin instruction manual, available at: http://www.bio-rad.com/webmaster/pdfs/9184_Chelex.PDF, last access: 24 April 2012, 1991.; Charrier, J. G. and Anastasio, C.: Impacts of antioxidants on hydroxyl radical production from individual and mixed transition metals in a surrogate lung fluid, Atmos. Environ., 45, 7555–7562, 2011.; Cho, A. K., Di Stefano, E., You, Y., Rodriguez, C. E., Schmitz, D. A., Kumagai, Y., Miguel, A. H., Eiguren-Fernandez, A., Kobayashi, T., Avol, E., and Froines, J. R.: Determination of four quinones in diesel exhaust particles, SRM 1649a, an atmospheric PM2.5, Aerosol Sci. Technol., 38, 68–81, 2004.; Damodaran, S.: Estimation of disulfide bonds using 2-Nitro-5-thiosulfobenzoic acid – limitations, Anal. Biochem., 145, 200–204, 1985.; Cho, A. K., Sioutas, C., Miguel, A. H., Kumagai, Y., Schmitz, D. A., Singh, M., Eiguren-Fernandez, A., and Froines, J. R.: Redox activity of airborne particulate matter at different sites in the Los Angeles Basin, Environ. Res., 99, 40–7, 2005.; Chung, M. Y., Lazaro, R. A., Lim, D., Jackson, J., Lyon, J., Rendulic, D., and Hasson, A. S.: Aerosol-borne quinones and reactive oxygen species generation by particulate matter extracts, Environ. Sci. Technol., 40, 4880–4886, 2006.; Connell, D. P., Winter, S. E., Conrad, V. B., Kim, M., and Crist, K. C.: The Steubenville Comprehensive Air Monitoring Program (SCAMP): concentrations and solubilities of PM2.5 trace elements and their implications for source apportionment and health research, J. Air Waste Manage., 56, 1750–1766, 2006.; Delhomme, O., Millet, M., and Herckes, P.: Determination of oxygenated polycyclic aromatic hydrocarbons in atmospheric aerosol samples by liquid chromatography-tandem mass spectrometry, Talanta, 74, 703–710, 2008.; DiStefano, E., Eiguren-Fernandez, A., Delfino, R. J., Sioutas, C., Froines, J. R., and Cho, A. K.: Determination of metal-based hydroxyl radical generating capacity of ambient and diesel exhaust particles, Inhal. Toxicol., 21, 731–738, 2009.; Eiguren-Fernandez, A., Shinyashiki, M., Schmitz, D. A., DiStefano, E., Hinds, W., Kumagai, Y., Cho, A. K., and Froines, J. R.: Redox and electrophilic properties of vapor- and particle-phase components of ambient aerosols, Environ. Res., 110, 207–212, 2010.; Eyer, P., Worek, F., Kiderlen, D., Sinko, G., Stuglin, A., Simeon-Rudolf, V., and Reiner, E.: Molar absorption co

 

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