World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Single-stranded Annealing Induced by Re-initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion Via Non-allelic Homologous Recombination, Volume 9

By Jinks-robertson, Sue

Click here to view

Book Id: WPLBN0003953162
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Single-stranded Annealing Induced by Re-initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion Via Non-allelic Homologous Recombination, Volume 9  
Author: Jinks-robertson, Sue
Volume: Volume 9
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Jinks-Robertson, S. (n.d.). Plos Genetics : Single-stranded Annealing Induced by Re-initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion Via Non-allelic Homologous Recombination, Volume 9. Retrieved from http://worldlibrary.net/


Description
Description : Copy number expansions such as amplifications and duplications contribute to human phenotypic variation, promote molecular diversification during evolution, and drive the initiation and/or progression of various cancers. The mechanisms underlying these copy number changes are still incompletely understood, however. We recently demonstrated that transient, limited re-replication from a single origin in Saccharomyces cerevisiae efficiently induces segmental amplification of the re-replicated region. Structural analyses of such re-replication induced gene amplifications (RRIGA) suggested that RRIGA could provide a new mechanism for generating copy number variation by non-allelic homologous recombination (NAHR). Here we elucidate this new mechanism and provide insight into why it is so efficient. We establish that sequence homology is both necessary and sufficient for repetitive elements to participate in RRIGA and show that their recombination occurs by a single-strand annealing (SSA) mechanism. We also find that re-replication forks are prone to breakage, accounting for the widespread DNA damage associated with deregulation of replication proteins. These breaks appear to stimulate NAHR between re-replicated repeat sequences flanking a re-initiating replication origin. Our results support a RRIGA model where the expansion of a re-replication bubble beyond flanking homologous sequences followed by breakage at both forks in trans provides an ideal structural context for SSA–mediated NAHR to form a head-to-tail duplication. Given the remarkable efficiency of RRIGA, we suggest it may be an unappreciated contributor to copy number expansions in both disease and evolution.

 

Click To View

Additional Books


  • Plos Genetics : Role of Transposon-deriv... (by )
  • Plos Genetics : Evolution of Mutational ... (by )
  • Plos Genetics : Actin Depolymerizing Fac... (by )
  • Plos Genetics : Dynein Regulates Epithel... (by )
  • Plos Genetics : Try-5 is a Sperm-activat... (by )
  • Plos Genetics : an Rna Transport System ... (by )
  • Plos Genetics : Identification of Novel ... (by )
  • Plos Genetics : a Flexible Approach for ... (by )
  • Plos Genetics : Dna Double-strand Breaks... (by )
  • Plos Genetics : Rhoa is a Modulator of t... (by )
  • Plos Genetics : Pathways of Distinction ... (by )
  • Plos Genetics : when Clocks Go Bad ; Neu... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.